An immunoassay for atrazine using tunable immunosorbent.
نویسندگان
چکیده
A novel, simple, economical, and environmentally friendly tunable immunosorbent-based immunoassay for sensitive and selective determination of atrazine is reported. Tunable immunosorbents consisting of a fusion between an elastin-like polypeptide made up of 77 repeating units of the pentapeptide VPGVG and a single-chain Fv of an anti-atrazine antibody were synthesized biologically and purified by temperature-triggered phase transition. A competitive immunoassay based on the competition of atrazine-horseradish peroxidase and atrazine was established with IC(50) and lower detection limit of 0.16 and 0.01 ppb, respectively. Excellent recoveries (mean values ranging between 92 and 104%) were demonstrated in simulated atrazine-contaminated water samples.
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عنوان ژورنال:
- Analytical biochemistry
دوره 322 2 شماره
صفحات -
تاریخ انتشار 2003